In gc, the detector responds to the thermal conductivity of the gas stream or the ionisation of the gas as it passes through a flame. Gas chromatography courses teach you how to use gas to separate different elements in a chemical mixture. Ashwani k sharma,department of biotechnology,iit roorkee. Learn more about the different separation techniques and the chromatography solutions that thermo fisher scientific offers. Gas chromatography continues to be one of the most widely used analytical techniques, since its applications today expand into fields such as biomarker research or metabolomics. Chromatography definitionchromatography is defined as physicalmethod of separation, in which themixture of analytes is separated using twophases, one is stationary phase and otheris mobile phase which percolates throughthe stationary phase. Nptel material is available for free on nptel website. Chromatography is based on the principle where molecules in mixture applied onto the surface or into the solid, and fluid stationary phase stable phase is separating from each other while moving with the aid of a mobile phase. Gas chromatography classification according to the force of separation 1 adsorption chromatography. Gas chromatography or gas liquid chromatography is a technique applied for separation, identification and quantification of components of a mixture of organic compounds by selective partitioning between the stationary phase and mobile phase inside a column followed by sequential elution of separated components. Postgraduate certificate in advanced chromatographic.
Gas chromatography runs on the principle of pa rtition chromatography for separation of components. Gain practical laboratory experience in gas chromatography. Bmc training provides a training course in applied gas chromatography in oil and gas training. Recommended books 4 books, please buy anything from the below links to support the channel. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. If you are not able to download lectures due to bandwidth constraints, you can obtain the courses in dvds by paying a nominal fee to cover the cost of storage media and distribution.
Find materials for this course in the pages linked along the left. Nptel syllabus novel separation processes video course. These include column chromatography, high performance liquid. The factors effective on this separation process include molecular characteristics related to adsorption liquid. Chromatography is a separation process that is achieved by distributing the components of a mixture between two phases, a stationary phase and a mobile phase. Analytical chemistry inorganic chemistry medicinal chemistry organic chemistry. This hazard can be present wherever combustible gases or vapors are in contact with a substance which will support combustion and the properties are in the explosive range. Online course basic analytical chemistry the university of tokyo via edx 0 151. Learn all you need to know about polymer chemistry through a novel. Semester wise suggested reading for civil engineering. At the end of the course the student would be able to handle the analysis of mg, ppm and ppb levels of analyte by appropriate instrumental methods. This new practical textbook enables the reader to make full use of gas chromatography.
Gas chromatography chemical engineering, iit bombay. A wide range of chromatographic procedures makes use of differences in size, binding affinities, charge, and other properties. In gas chromatography, the moving phase or mobile phase is a carrier gas, usually an inert gas such as helium or an unreactive gas such as nitrogen. Here are the links to the free online e course on gc. The film must have a high boiling point mobile phase. Gas chromatography laboratory training courses on hplc. The course provides the fundamentals to understand the techniques and instrumentation involved in this powerful analytical tool. For the love of physics walter lewin may 16, 2011 duration. Gas chromatography is a method of chemical analysis performed by separating and examining vaporized compounds. Supercritical fluid chromatography is one of the most important column chromatography methods after gas chromatography gc and highperformance liquid chromatography hplc. The troubleshooting and maintenance guide for gas chromatographers. Like the video and subscribe to channel for more updates. By the end of this course, you will feel comfortable understanding any modern gc.
Develop a greater understanding of the chromatographic technique your laboratory uses. However, with hplc, its a more advanced technique in that youre working with very, very small quantities, and the detector in the machine is much more sensitive. Explore the full program of hplc, gc and sample prep training courses for 2020. Nptel syllabus analytical technologies in biotechnology. The stationary phase is a liquid layer supported over a stationary phase while the mobile phase is an inert and stable gas. Lecture series on industrial instrumentation by prof. Learn for free, pay a small fee for exam and get a certificate. Chromatography not only separates out your target molecule, it concentrates it and offers a variety of downstream applications as a result. This type of chromatography was primarily designed to evaluate volatile. Thus the lectures will offer a substantial amount of information to the students.
The last kind of chromatography is gas chromatography. Introduction to gas chromatography course and its objectives the overwhelming response to the hplc free elearning course has encouraged me to move ahead with the other courses announced earlier. Chromatography biochemistry of medics 1 slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Chemical analysis of hazardous materials, environmental samples, inorganic, organic and biomaterials at trace and ultra trace quantities. Advanced membrane technology and applications by n n li wiley. The power of chromatography comes from its ability to separate a mixture of compounds, or analytes, and. Join our civil and construction engineering training courses, electrical and power engineering training courses and. Nptel mechanical engineering video lecture online study. Gas chromatography differs from other forms of chromatography in that the mobile phase is a gas and the components are separated as vapors it is thus used to separate and detect small molecular weight compounds in the gas phase. This course is designed for beginners, intermediates, and even advanced level users in gc who want practical laboratory experience and thorough understanding of gc fundamentals. The stationary phase is a microscopic layer of liquid or polymer on an inert solid support, inside a piece of glass or metal tubing called a column a homage to the fractionating column used in. The instrument used to perform gas chromatography is called a gas chromatograph or aerograph, gas separator. This postgraduate certificate in advanced chromatographic techniques was developed based on an analysis of the importance of the pharmaceutical and related industries in ireland and within the southeast region and also using information generated from th. The modern analytical techniques with high accuracy like high performance liquid chromatography hplc and gas chromatography gc is extensively discussed in the book to get the understanding of.
This article throws light upon the twelve types of chromatographic techniques used in biochemistry. The stationary phase is a microscopic layer of liquid or polymer on an inert solid support, inside a piece of glass or metal tubing called a column a homage to the fractionating column used in distillation. In column chromatography, mobile phase flows through the packed column, while in thin layer chromatography, mobile phase moves by capillary action. Gc online training laboratory training courses on hplc. Chapter 1 introduction, chromatography theory, and instrument calibration 1. Search for newer sources is of continued interest to dyers. Nptel provides elearning through online web and video courses various streams.
Chromatography is a field of chemistry with many practical applications, such as in forensics, environmental testing, explosives detection and other laboratory technology fields. Chapter 1 introduction, chromatography theory, and. Practical gas chromatography a comprehensive reference. This fourth edition of the classic guide for every user of gas chromatographic instrumentation is now updated to include such new topics as fast gc using narrow, short columns, electronic pressure control, and basic aspects of quantitative gas chromatography. Nptel syllabus natural dyes video course course outline dyeing fabric is an ancient art. Alok barua, department of electrical engineering,iit kharagpur. Analytical technologies in biotechnology online course. Classes specifically in gas chromatography are rare, but this subject is commonly taught in broader chemistry courses.
Top 12 types of chromatographic techniques biochemistry. The sample is either a gas or a liquid that is vaporized in the injection port. Flue gas analysis can be used to detect and to confirm and locate suspected condition of this nature. Lecture notes polymer physics materials science and. Hplc instrumentation, solvent reservoir in hplc, degasser in hplc, mixing valve in hplc, hplc pump, precolumn in hplc, guard column in hplc, sample injection in hplc, analytical column in hplc. Lecture 35 introduction to chromatography i by nptel iit. These courses often include a laboratory component and teach you how to use gas chromatography machines. Nevertheless, the material should be useful as an overview of the subject for those. Sfc combines the advantages of gc and hplc thanks to the superior properties of supercritical fluids. The use of flue gas analysis to promote efficiency are varied and many. Mudakavi,department of chemical engineering, iisc bangalore. Nptel, online courses and certification, learn for free.